In this experiment we captured the dynamics of hemicylindrical micelles - formed in a 2mM CTAB solution - migrating across the surface of HOPG. The micelles are normally quite stable so we perfuse...
Collagen is the most abundant protein found in humans and other animals. It assembles in vivo into hierarchical structures found in numerous tissues. In addition, it is widely used to create substr...
GetStarted is an intuitive feature in the Asylum AFM software that automatically determines the optimal imaging parameters for rapid, simple AFM imaging. It uses conventional tapping mode (AC mode)...
You may be a biologist new to the AFM or an AFM expert starting to study biology. When you first start out, using an AFM for biological applications can seem overwhelming. Although there are challe...
Miniaturization of cantilevers for Atomic Force Microscopy has increased their resonant frequencies and decreased their thermal noise, allowing faster, lower noise measurements. When used in the ex...
This movie shows a polymer thin film consisting of syndiotactic polypropylene (sPP) and polystyrene (PS) that was imaged as a function of changing temperature on the Cypher ES AFM using blueDrive p...
This movie shows a polystyrene - polybutadiene - polystyrene triblock copolymer film annealing during exposure to toluene solvent vapor as imaged with the Cypher ES AFM. When films are originally f...
A blend of polystyrene (PS) and polypropylene (PP) was heated to 140°C to melt the syndiotactic PP and then cooled at a constant rate while imaging at one frame per minute. As the sample cools, the...
Movie showing the shape recovery of a polystyrene (PS) microparticle during annealing. Imaged using tapping mode on a MFP-3D with scan size 15 μm. The PS particle was flattened with a high-temperat...
This movie was taken on the Cypher Environmental AFM using blueDrive photothermal excitation. The freshly cleaved calcite sample was imaged under continuous flow (~3uL/s) of growth and etch solutio...
This movie of 30 individual frames shows point defects on the cleavage plane of calcite imaged in water. If you watch different parts of the image you can see individual defects appear, disappear, ...
This movie of 26 frames shows individual defects moving around in the two-dimensional crystal formed by the membrane protein Bacteriohodopsin (purple membrane). If you watch the "clouds" moving aro...
This movie shows the sublimation of a single crystal of anthracene in air. Anthracene is an organic molecule consisting of three fused benzene rings; it is solid at room temperature, but volatile e...
The Cypher AFM from Asylum Research was released at MRS 2008 in Boston. It is the world's highest resolution AFM and is the first totally new small sample AFM in over 10 years.
Closed loop AC Mode image in air of atomic steps on HF-etched Mica taken with the Cypher AFM using a 10µm cantilever with a resonance frequency of 4.6 MHz. 40Hz line rate. 512 x 512 pixels. Video i...
These two movies (at 0.6 and 1.2 frames per second) show the dynamics of loosely bound DNA in liquid (Z range, 2nm). The sample preparation used a lower than normal concentration of the divalent ca...
The surface of a freshly cleaved calcite crystal in humid air reconstructs on the time scale of minutes to hours. The reconstruction is known to be water driven, but there is still speculation abou...
Celgard is an interesting material that is used as a porous battery separator membrane and as a filtration membrane in medical applications. It is made by stretching isotactic polypropylene. The to...