yt:channel:UCWkjWb52NtKzZn8CGSRL67g UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2011-06-10T19:38:03+00:00 yt:video:_jTpvU72fvQ _jTpvU72fvQ UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2017-03-17T17:42:25+00:00 2017-04-27T07:40:20+00:00 Dynamics of Micelles captured @ 2.4 fps with the Cypher VRS Video-Rate AFM In this experiment we captured the dynamics of hemicylindrical micelles - formed in a 2mM CTAB solution - migrating across the surface of HOPG. The micelles are normally quite stable so we perfused in a small amount of isopropyl alcohol to induce dynamics. For these movies we acquired images (200 x 200 pixels) at 2.4 frames per second. In order to visualize the dynamics more clearly the playback rate is 6X faster than the acquisition rate. yt:video:K-bBdciWpXI K-bBdciWpXI UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2017-02-02T21:22:56+00:00 2017-05-06T20:54:58+00:00 DNase Cleavage of Lambda DNA yt:video:WS2IMdY5N5U WS2IMdY5N5U UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2017-02-02T21:02:52+00:00 2017-07-14T20:54:07+00:00 Assembly of Collagen Fibrils on Mica Collagen is the most abundant protein found in humans and other animals. It assembles in vivo into hierarchical structures found in numerous tissues. In addition, it is widely used to create substrates and 3D scaffolds in vitro for cell and tissue culture. Hence, understanding the mechanism of collagen self-assembly has both fundamental and practical importance. Instrument used: Cypher VRS Video-Rate atomic force microscope, (the first and only full-featured video-rate AFM available today). See website for imaging results description and to see more on video-rate imaging: www.oxford-instruments.com/cypher-vrs yt:video:fEqlsE9co8M fEqlsE9co8M UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2015-08-24T23:44:43+00:00 2017-07-25T18:06:03+00:00 Webinar - "Beyond Topography: New Advances in AFM Characterization of Polymers" Presented on May 28, 2015 by Dr. Donna Hurley, Lark Scientific and Dr. Anna Kepas-Suwara, Tun Abdul Razak Research Centre (TARRC) Whether investigating fundamental research principles or engineering a specific product, the atomic force microscope (AFM) is a key instrument for evaluating polymers and polymer blends. Its spatial resolution enables visualization of sub-micrometer and sub-nanometer morphology and structure. However, recent advances mean that AFMs can also measure the physical properties and functional behavior of polymers at small length scales. In addition to familiar topographic imaging, AFMs can probe molecular-level forces; map mechanical, thermal, and electrical properties; and assess solvent and thermal effects in near real time. This webinar provides an overview of the AFM’s powerful capabilities for polymers characterization and will cover: • AFM methods for fast topographic imaging, even in liquids and at high temperatures • Recent advances in viscoelastic measurements • Nanomechanical mapping of rubber blends • AFM techniques to probe electrical and functional behavior yt:video:XWFSiCz7QlA XWFSiCz7QlA UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2015-07-22T00:34:44+00:00 2015-10-29T07:28:33+00:00 GetStarted - Automated Operation for Tapping Mode Imaging in Air GetStarted is an intuitive feature in the Asylum AFM software that automatically determines the optimal imaging parameters for rapid, simple AFM imaging. It uses conventional tapping mode (AC mode), so imaging is gentle on the tip and sample. It's fully compatible with the wide range of operating modes that use tapping mode. GetStarted is superior to features that claim to optimize imaging because GetStarted uses a predictive algorithm that enables quality data from the very first scan line as shown in the DNA image to the right. GetStarted is available exclusively on the MFP-3D Infinity™ and Cypher™ AFMs. yt:video:Wp-U5HXbMys Wp-U5HXbMys UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-11-03T20:33:50+00:00 2017-04-26T01:08:13+00:00 GetReal - Automated Probe Calibration yt:video:rJdC-zK79o8 rJdC-zK79o8 UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-10-20T23:43:30+00:00 2016-09-22T05:33:46+00:00 Melting and recrystallization of a sPP-PS polymer film This movie shows a polymer thin film consisting of syndiotactic polypropylene (sPP) and polystyrene (PS) that was imaged as a function of changing temperature on the Cypher ES AFM using blueDrive photothermal excitation. The scan size is 3 µm and the data channel shown is phase because it shows the most contrast. The round, isolated domains are PS and the continuous matrix around them is the sPP. Note that the melting temperature of sPP is ~ 130-170°C while the melting temperature of PS is ~240°C. The movie starts with the sample at ~60°C. It is then slowly heated to ~135°C, during which time you can observe the PP crystallites melt, diminishing in size and forming a melted layer. You can actually see the high motility of the crystallites in the melt. Notably the heating is stopped before the sPP is fully melted and the sample is then slowly cooled. The remaining crystallites act as nucleation sites, recrystallizing and growing. Once the temperature has returned to 60°C, you see larger features appear. Note that the recrystallization occurs much differently if the sPP is fully melted. This case can be seen in another movie here http://www.asylumresearch.com/Gallery/Movies/Movie23.shtml. yt:video:eHMEK-kUOl8 eHMEK-kUOl8 UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-10-20T23:40:34+00:00 2017-05-11T06:16:40+00:00 Annealing of a PS-PB-PS block copolymer in toluene solvent vapor This movie shows a polystyrene - polybutadiene - polystyrene triblock copolymer film annealing during exposure to toluene solvent vapor as imaged with the Cypher ES AFM. When films are originally formed by spin casting from solution, block copolymers are known to organize into various microphase morphologies. However this morphology might not be the lowest energy state, so annealing often results in significant restructuring. The movie begins just as toluene vapor enters through the closed cell. These are 4um scans, so you can see not only changes to the microstructure but also near the end a couple holes open up as the film delaminates from the silicon substrate. We then digitally zoom into a smaller area and examine the microphase morphology changes in more detail. yt:video:w4fzD-EvLOw w4fzD-EvLOw UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-09-26T14:15:50+00:00 2015-11-09T23:05:02+00:00 Recrystallization of polypropylene in polystyrene / polypropylene blend A blend of polystyrene (PS) and polypropylene (PP) was heated to 140°C to melt the syndiotactic PP and then cooled at a constant rate while imaging at one frame per minute. As the sample cools, the continuous PP phase first nucleates and then forms partly ordered, semicrystalline regions. Some of the regions form on top of the PS spherical domains. Imaged with the Cypher ES AFM; scan size 4 μm. yt:video:iOmteePiapE iOmteePiapE UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-09-25T18:52:05+00:00 2016-05-12T23:03:36+00:00 Annealing of Shape Memory Polymer Movie showing the shape recovery of a polystyrene (PS) microparticle during annealing. Imaged using tapping mode on a MFP-3D with scan size 15 μm. The PS particle was flattened with a high-temperature, high-pressure nanoimprint lithography (NIL) process and then thinly coated with gold. The movie starts with the particle heated to 80°C. The temperature is rapidly stepped to 102°C, then over the course of three hours the temperature is gradually increased to 110°C. During annealing the particle diameter decreases and the height increases, recovering the original, pre-NIL spherical shape. A surface wrinkling morphology develops that gives information on recovery dynamics and strain energy release. Applications for micro- and nanoscale polymer particles include drug delivery and electronic packaging; incorporating shape memory effects could lead to many more. Data courtesy of Jason Killgore (NIST) and Lewis Cox (University of Colorado-Boulder). Adapted from L.M. Cox, J.P. Killgore, Z. Li, Z. Zhang, D.C. Hurley, J. Xiao, and Y. Ding, Adv. Mater. 26, 899 (2013). yt:video:lQOciQIlHqs lQOciQIlHqs UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-07-10T22:44:17+00:00 2016-12-21T06:53:05+00:00 Contact Resonance Tools for AFM Nanomechanics Part II of the Nanomechanics Series: Opportunities, Challenges and Frontiers in Nanomechanical Measurements with AFM Donna Hurley, National Institute of Standards & Technology Roger Proksch, Asylum Research, an Oxford Instruments Company Nanoscale information on mechanical properties is critical for many advanced materials and nanotechnology applications. Atomic Force Microscopy techniques for probing mechanical properties of samples in the nanometer range have emerged over the past decades. In contrast to the large number of techniques for softer samples, few techniques are capable of measuring moduli in the 1-200 GPa range. One technique, Contact Resonance (CR), has proven to work very well in this range. CR methods operate in contact mode with dynamic excitation near a cantilever resonant frequency, enabling sensitive measurements over a wide range of materials. Moreover, analysis of the CR peak frequency and quality factor yields accurate, quantitative data on elastic modulus and viscoelastic damping. In this webinar, we'll explain the basic concepts of measurements with different CR approaches including: • Point spectroscopy • Qualitative contrast imaging • Quantitative mapping We'll also discuss practical implementation of contact resonance to a variety of samples and some of the pitfalls and artifacts you might encounter. Finally, we'll present results on how CR methods have been used to improve understanding of systems such as: • Composites • Thin films • Biomaterials • Polymer blends The nanomechanical characterization capabilities of CR methods, as you will come to learn, are an essential tool for the development, production, and in-situ monitoring of today's and tomorrow's materials. This webinar was first present live on June 26, 2013 by Dr. Donna Hurley and Dr. Roger Proksch. Donna Hurley Dr. Hurley leads the AFM Nanomechanics Project in the Material Measurement Laboratory at the National Institute of Standards & Technology (NIST) in Boulder, CO. Her project team creates and applies AFM measurement technology for material-property characterization. For over 10 years, she has developed contact resonance AFM modes for quantitative nanomechanical imaging. She is author or co-author of numerous technical articles, including chapters in the recently released Scanning Probe Acoustic Techniques (Springer-Verlag, 2012) and the upcoming SPM in Industrial Applications: Nanomechanical Characterization (John Wiley & Sons, 2013). She has a Ph.D. in Physics from the University of Illinois at Urbana-Champaign. Prior to NIST, she worked at GE Corporate Research (Schenectady, NY) and the University of Nottingham (UK). Roger Proksch Dr. Proksch is President and co-founder of Asylum Research, an Oxford Instruments company. He has over 20 years of AFM experience. He has co-authored many papers and is a co-inventor on numerous AFM patents. He received his Ph.D. from the University of Minnesota. yt:video:KH3B0JTzLHE KH3B0JTzLHE UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-05-27T17:23:12+00:00 2017-07-15T00:47:09+00:00 Getting Started with AFM in Biology -- It's Easier Than You Think You may be a biologist new to the AFM or an AFM expert starting to study biology. When you first start out, using an AFM for biological applications can seem overwhelming. Although there are challenges for successful AFM in biology, we'll show you it's easier than you think! Sample prep is a critical part of successful Bio-AFM. There are some basic principles that help insure success. However, life is complex and so are biological samples -- with variations as large as the number of researchers. Thus, at the same time, you will need to be flexible -- your samples may require a prep that is tweaked and tailored a bit to optimize your results. Working in liquid adds another challenge. In addition, the choice of measurement mode may not be obvious. Do I want to use tapping mode? Force curves? Contact Mode? Finally, there is a long list of commercial cantilevers available and choosing the best one can be like looking for a needle in a haystack. In this webinar we will present four case studies of a few typical biological samples: 1. Imaging DNA in liquid -- including routine helix resolution 2. Imaging living cells in liquid 3. Measuring Young's modulus of living cells 4. Unfolding forces in Titin In each case we will discuss sample prep, lever and measurement mode choice and follow up with data interpretation and cautionary examples of experimental artifacts. The goal of this webinar is to give you the confidence to repeat these experiments yourself and then extending them to fit your own research. This webinar was first presented live May 22, 2013 by Dr. Irene Revenko. Irene is one of the world's leading experts in Bio AFM. She is a staff scientist at Asylum Research and has over 19 years of AFM experience. She initiated the first bio-classes at Asylum Research in 2002 and since then has taken many students from their first AFM measurements through cutting edge results. yt:video:xkQjYzRzF5U xkQjYzRzF5U UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-05-12T15:46:44+00:00 2016-05-20T14:23:25+00:00 Introduction and Innovations in High Speed Quantitative Nanomechanical Imaging Part I of the Nanomechanics Series: Opportunities, Challenges and Frontiers in Nanomechanical Measurements with AFM This presentation will begin with a survey of the mechanical properties that can be investigated with the wide array of both old and new nanoscale property mapping techniques available to materials scientists. We will then introduce two new techniques for nanomechanical studies that allow unambiguous interpretation of material properties: AM-FM and Loss Tangent. Amplitude-modulated (AM) atomic force microscopy, also known as tapping mode, is a proven, reliable and gentle imaging method with wide spread applications. Previously, the contrast in tapping mode has been difficult to quantify. The new AM-FM imaging technique combines the features and benefits of normal tapping mode with the quantitative, high sensitivity of Frequency Modulation (FM) mode. Loss Tangent imaging is another recently introduced quantitative technique that recasts the interpretation of phase imaging into one term that includes both the dissipated and stored energy of the tip sample interaction. These techniques allow high speed, low force imaging in tapping mode while providing quantitative Elasticity and Loss Tangent images. This webinar in our Nanomechanics Webinar Series was first presented live March 23, 2012 by AFM pioneer, inventor and Asylum Research co-founder, Dr. Roger Proksch. To view our other webinars and sign up to be notified about future webinars please visit http://www.AsylumResearch.com/webinars yt:video:S_DR_CO_BPE S_DR_CO_BPE UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-05-06T17:10:13+00:00 2017-06-02T04:43:43+00:00 Smaller and Quieter: Ultra-High Resolution AFM Imaging Miniaturization of cantilevers for Atomic Force Microscopy has increased their resonant frequencies and decreased their thermal noise, allowing faster, lower noise measurements. When used in the extremely low-noise Cypher AFM, these levers have enabled significant improvements in imaging resolution in air and especially in liquids. On crystals, individual atomic point defects can now be routinely resolved and this higher resolution also extends to biological samples. Examples shown include the movement of individual point defects in bacteriorhodopsin, atomic point defects in calcite, and resolution of the double-helix structure of DNA in solution. This webinar was first presented live February 22, 2012 by AFM pioneer, inventor and Asylum Research co-founder, Dr. Jason Cleveland. To view our other webinars and sign up to be notified about future webinars please visit http://www.AsylumResearch.com/webinars yt:video:kpThWs8dWOI kpThWs8dWOI UCWkjWb52NtKzZn8CGSRL67g AsylumResearchVideo https://www.youtube.com/channel/UCWkjWb52NtKzZn8CGSRL67g 2014-01-21T01:03:37+00:00 2017-06-29T22:00:13+00:00 Calcite Screw Dislocation Under Continuous CaCO3 Growth-etch Solution This movie was taken on the Cypher Environmental AFM using blueDrive photothermal excitation. The freshly cleaved calcite sample was imaged under continuous flow (~3uL/s) of growth and etch solution. The pH of the solution was varied to control the growth/etch rate. Cantilever: Arrow UHF Mode: AC Mode in water Excitation: blueDrive at 525 kHz ScanRate: 19.5lines/s ScanSize = 500nm Points/Lines: 256/128 Imaging Time per Frame = 6.55 seconds Playback rate = 52.4X acquisition rate http://www.asylumresearch.com/Products/blueDrive/blueDrive.shtml