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This video is unlisted. Only those with the link can see it. Ion Torrent Chemistry

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Published on Jun 5, 2010

Ion Torrent pairs semiconductor technology with a simple sequencing chemistry that is based on a well-characterized biochemical process. In nature, when a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion is released as a byproduct. Ion Torrent uses a high-density array of micro-machined wells to perform this biochemical process in a massively parallel way. Each well holds a different DNA template. Beneath the wells is an ion-sensitive layer and beneath that a proprietary Ion sensor. Here's how the technology is used to call a base: If a nucleotide, for example a C, is added to a DNA template and is then incorporated into a strand of DNA, a hydrogen ion will be released. The charge from that ion will change the pH of the solution, which can be detected by our proprietary ion sensor. Our sequencer—essentially the world's smallest solid-state pH meter—will call the base, going directly from chemical information to digital information. The Ion Personal Genome Machine (PGM™) sequencer then sequentially floods the chip with one nucleotide after another. If the next nucleotide that floods the chip is not a match. No voltage change will be recorded and no base will be called. If there are two identical bases on the DNA strand, the voltage will be double, and the chip will record two identical bases called. Because this is direct detection—no scanning, no cameras, no light—each nucleotide incorporation is recorded in seconds.

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Top Comments

  • Tom Howard

    Who noticed that the middle 4 'symbols' below the IonTorrent logo belong on a PlayStation controller?

    · 8

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  • Achillies11ify

    Don't forget that the core technology was not developed by Rothberg (Ion torrent), his company has a non-exclusive license from the true inventor Prof Chris Toumazou

    · 5

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All Comments (24)

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  • Tam Nguyen

    Do you know any University in Euro training course for applying Ion Torrent System? I am a animal researcher, major on breeding.

    ·

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  • Guitarristandgoats

    Thanks :D still cool

    ·

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    in reply to clicliful (Show the comment)
  • clicliful

    not exactly: in a microwell there are multiple copies of an identical dna strand. Each strand expells one proton, so the pHmeter detects a change in proton concentration. Anyway, the sensitivity is really impressive! :)

    ·

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    in reply to Guitarristandgoats (Show the comment)
  • Guitarristandgoats

    gimme 40 of those!

    ·

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  • Guitarristandgoats

    Fuck, that uses a sensor which can detect ONE single proton? D:

    ·

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  • GAO yang

    not exactly same. PS has a delta instead of the plus sign on IonTorrent

    ·

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    in reply to Tom Howard (Show the comment)
  • zigaudrey

    0:47 The green tache look like pac man

    ·

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  • zigaudrey

    Yes,Me too!

    ·

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    in reply to Tom Howard (Show the comment)
  • UltraNationalist Viktoria

    Si mi Sussio. Si.

     Das Fuhrer

    ·

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    in reply to karen0133 (Show the comment)
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