pGLO Transformation

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Uploaded by on Apr 29, 2011

Bio Rad's pGLO transformation kit is demonstrated in this video. The pGLO plasmid has 3 genes spliced into it. Bla is a gene that codes for a protein that nullifies the antibiotic ampicillin. Ara C is a gene that codes for a protein that turns on the GFP gene. The GFP gene codes for the green fluorescence protein (GFP), which glows green in the presence of UV light. The GFP is normally found in the jellyfish Aequorea victoria, which makes it bioluminescent. The pGLO plasmid is inserted into E. coli bacteria, which makes the bacteria survive in ampicillin and glow green in UV light.

ScienceFix.com
Producer: Darren Fix

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Education

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Uploader Comments (sciencefix)

  • Heat shock for 50 seconds.

  • Nope, I recently uploaded all of my old Google Video videos, since they are shutting down the service soon.

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  • Fucken Magnets! How do they work?!

  • for how long do you heat shock the bacteria?

  • umm....did you steal this video?

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