Recombinant DNA ©2004 Demonstratives, Inc.

A 3D animation illustrating the process by which a protein is mass-produced using spliced DNA and bacterial replication. For more animation samples, please visit:
www.demonstratives.com.

Category:

Science & Technology

Tags:

• DNA
• bacteria
• protein
• recombinant
• ampicillin
• demonstratives

[cheddybumpkins]

RBS = ribosomal binding site? I thought that the ribosomes can only read mRNA, unless...does the ribosome follow the DNA polymerase as it tracks down the operon and translate continuously?

[scatterbrain73]

@cheddybumpkins That's right. The RBS designation here refers to the segment of DNA that encodes for the RBS within the messenger RNA. We basically skipped over the translation step.

[ramprs26]

what is a fusion protein ?and what does RBS stand for???

[scatterbrain73]

@ramprs26 A fusion protein is created by the joining of two separate strands of DNA originally coded to make two separate proteins. I forget what RBS stands for. I'm just the animator.

[Djiniiiiii]

@ramprs26 Most likely, RBS stands for Ribosome Binding Site. That's the sequence where ribosome binds to the mRNA molecule to begin searching for the first Start codon (AUG), so that it just doesn't randomly pick a different AUG codon in the mRNA and makes a different protein.

[scatterbrain73]

@Djiniiiiii Yeah, I think you're right.

[1up7up]

whew, this video helped me on my biology homework

many thanks for the upload

[Thalyvanhoff]

oh yeah!!

[KatariaGujjar]

@scatterbrain73

RBS = ribosomal binding site

[maxcope540]

great illustration! must more clear seeing this happen visually then just being told about it step by step...