Shotgun sequencing

http://www.bimatics.blogspot.com


In shotgun sequencing many copies of the entire genome are blown up into millions of small fragments. Each small fragment is sequenced. Powerful computers then assemble the individual fragments into the original configuration. Repeat sequences pose a problem for this approach because their sizes can be much larger than the small fragments.

Category:

Education

Tags:

• bioinformatics
• dna
• rna
• protein
• gene
• genomics
• proteomics
• shortgun
• sequencing

[howtoshyne]

Does anybody else find it amusing that the big bad private company had to rely on the "public data" to finish? ;)

[christopheradair]

@liner986 primer walking or by filling them in via PCR. This can only be used for small gaps though... more of an educated guess type of deal.

[liner986]

so how do you complete the sequence the "left out" fragment ends that the computer cant figure out by overlapping?

[eucalyptus321123]

hah interesting, i just discussed the repeat problem with a prof of mine. awefully tiring work of completing whole genome sequences

[SpetsnazOXB]

I love genetics

[gtyellow435]

first comment post woohoo