ISSCR 2012 -- Leaving Nothing to Chance

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Induced pluripotent stem cells (ipscs) offer a new cellular window into disease and therapy. Traditional reprogramming and transgenic methods can disrupt gene function through random DNA insertion. Dr. Mark Tomishima, of the Sloan Kettering Institute Stem Cell Research Facility, discusses the work of his lab in evolving their methods to reprogram cells using Sendai viral vectors that do not alter the genome. They now also target transgene insertion through the use of zinc finger nucleases. These two technologies allow the production of engineered iPSC lines that lack randomly inserted DNA.

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[Neueregel]

nice DNA techniques