Real Time QPCR Data Analysis Tutorial (part 2)
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After we calculate the fold difference in expression between the control sample and the tested samples, how could we know that the increase or decrease in expression is significant?
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Great video
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Thanks a lot.... great help.
Very simple explanation of difficult concept.
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How do you measure efficiency?
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what about fold if it is less than 1? for example, by Livak method, we found 2^-ΔΔCt as 0,300. what does it mean?
ufukufi 2 weeks ago
@ufukufi Typically, it is very difficult to obtain statistically significant differences with fold changes below 1. However, the p-value will set you free!
americanbiotech 2 weeks ago
@waweel, Once you have the data you need to analyze it using standard statistical methodology. Which method you use really depends on a number of factors including how your experiment was run, how many samples were included and how many variables are being analyzed.
americanbiotech 2 months ago