Composite phase contrast/florescence video of a membrane viewed in a face-on configuration. Approximately 1 particle out of 30,000 particles is fluorescently labeled. Phase contrast and florescence images are acquired simultaneously and are subsequently overlaid with nanometer precision. Fluorescently labeled rods within the membrane are viewed along their axial directions and appear as green isotropic spots. The diameter of the membrane shown is
15 μm. The video is sped up by a factor of 2.
This video appeared in
E. Barry and Z. Dogic, PNAS, 107, p.10348-10353 (2010)
More Information:
http://www.pnas.org/content/107/23/10348.full
http://www.pnas.org/content/107/23/10348/suppl/DCSupplemental
Email: dogiclab@gmail.com
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