Bacteriophage lambda lysis and lysogeny

Microscopic movie of E.coli infected with phage lambda. Infected cells turn green. One infected cell pops (i.e., lysis). A second infected cell goes dormant (i.e., lysogeny). Movie by Francois St-Pierre at MIT, as published in research paper PMID: 19098103.

Category:

Science & Technology

Tags:

• bacteriophage
• lambda
• lysis
• lysogeny

[shafro1]

what is the time scale of the video? how long in between pictures. thanks

[chthonicphage]

@shafro1

The overall movie covers ~8 hours. You can see a time lapse "film strip" with time noted in minutes in the original research paper.  See Figure 4 of the original research paper ("Determination of cell fate selection during phage lambda infection") <-- just do a web search on the title of the paper, I'm not sure how to link a URL directly in these comments.

[skagsux]

Which gene is GFP tagged?

[chthonicphage]

Constitutive E.coli promoter inserted into a non-coding sequence. More info. in the paper cited in the video's description.

[babertels]

This is incredible. Thanks for helping me to visually understand this process. It is easy to understand the theory behind something, but to actually see how is works, is always cool.