Import SRA of ChIP-seq data through DRA & Galaxy

The NGS generates huge data and you need a high spec computera with a very large storage and RAM. But, of course, only limited biologists are taking such an advantage. Recently, more and more NGS data are submitted to GEO database, how to make use of them?

NCBI provides NGS data in SRA format, though many analysis software takes FASTQ files as inputs. If you have some skills, it's not very difficult to extract FASTQ files from an SRA file, but it's not easy for many biologists.

In this movie tutorial, I show you how to get FASTQ files of a series which you find in GEO, and analyze them without high spec machines nor difficult software tools. Finally you can get results of peak calling files, which data size are significantly small and you can handle them on any PC or Mac, to import Subio Platform for biological exams.

Category:

Science & Technology

Tags:

• ngs
• chip-seq
• analysis
• software
• tutorial
• geo
• sra
• fastq
• dra
• galaxy

[songdaowen]

really helpful. Thank you!