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Ph.Diva and the Mystery Band - Animation of Life in the Biotech lab

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Published on Mar 26, 2012

See all Ph.Diddy videos here:
- "Ph.Diddy is on the scene" - http://youtu.be/lCblncsE8wQ
- "Ph.Diddy at the Conference" - http://youtu.be/ajdbuj8P784
- "Behind the scened at Halloween with Ph.Diddy": http://youtu.be/r_NdyX70MF8 PCR

April Brite (aka the Ph.Diva) is a biotech Postdoc. April is thrilled when her abstract is picked for an oral presentation, and starts preparing her presentation. When her PCR does not go exactly as planned, she sets off on a journey filled with frustrations, anticipation, fascination and determination. Will our Ph.Diva make it in time for her conference?

Acknowledgements: This video is inspired by all the dedicated everyday-heroes-of-science. Applied Biosystems recognizes your passion and admires your perseverance!

BY POPULAR DEMAND - the song is now available on iTunes!
http://itunes.apple.com/us/album/ph.-...

For more information on PCR, visit www.lifetechnologies.com/elevatepcr

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Lyrics:

There's just three weeks left until the conference deadline
My next post-doc and funding's on the line
I just got off a call with a great opportunity
They rate my work so much, they want it given verbally!

Need that last gel image so I don't come unstuck
Get the thermal cycler going, and tap the lid for luck
My RT-PCR hasn't quite gone as planned
As there on the gel.... is a total mystery band!

The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Something here defies explanation!
The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Discovery through experimentation!

I've checked for DNA and these primers of mine
Without reverse transcriptase everything's just fine
Are my primers forming dimers, or is something worse about?
Red faced I clean my bench, so there's no shadow of doubt...

The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Something here defies explanation!
The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Discovery through experimentation!

With everything ruled out, I run it again so I can see
And the same two bands are staring back at me!
But when my product's sequenced I soon realize:
I can lock down my presentation, 'coz it's an alternative splice!

The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Something here defies explanation!
The pressure builds again - ANTICIPATION
Curiosity game - A FACINATION
Results to obtain - DETERMINATION
Discovery through experimentation!

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