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Zinc Fingers: TFIIIA Bound with DNA

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Uploaded by on Jun 30, 2007

Transcription factor IIIA contains several repeat sequences of the motif with two cysteine and two histidine residues as ligands to bind a zinc atom. In this movie, the N-terminal six fingers of Xenopus TFIIIA bound to 5S rRNA gene are shown. The structure is from 1TF6.pdb (ref. Nolte et al. PNAS v95, 2938).

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  • The zinc finger motif was discovered from alignment of Cys..Cys..His..His binding to zinc, the residues between Cys and His forming a finger structure. You can read a review by Rhodes and Klug in Scientific American vol. 268, p. 32 (1993).

  • How were ZFN discovered?

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