Making a Wet Mount for Microscopy
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Uploader Comments (microbehuntercom)
Top Comments
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thumbs up if ur using this for home work :D
5 months ago 4
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This video is Absolutely brilliant...best video about slide making on youtube :P
All Comments (14)
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helped me a lot! thankss :)
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How do you get minimal air bubbles
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Thanks you helped I got a 100-900x microscope
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Oh! Kind of like immersion oil usage for the 100x objectives. Hmm. That makes sense... sort of. I'll have to test this out to see for myself when I can get my hands on a microscope (hopefully this week at a college lab). I'm hoping that a 40x (or anything higher than that, but below 100x oil) objective lens will show the difference in resolution (not depth of field, but what I'm assuming to be actual detail difference, or sharpness... e.g. resolution) of the specimen. Thx
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i have a exam tomorrow and this help me a lot THANKS MATE :)
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@ 6:39 - 6:46 "..otherwise you won't get the optimum RESOLUTION."
Could you clarify this point a little more?
Very informative video! Great techniques I have never seen. I especially like the last technique for larger/thicker specimen.
huyked 8 months ago
@huyked The mounting medium (water) is very important. The effect of the water is stronger the higher the numerical aperture of the objective (for low N.A., such as the 4x objective, this is pretty irrelevant). Without water many light rays from the specimen do not reach the objective, because they are bent away. There are simply too many air-glass transitions. With water more light rays (= more information) is able to reach the objective and the resolution as well as the brightness are higher.
microbehuntercom 8 months ago