Cutting with a microtome

Tissue cutting with a AO rotary microtome

Category:

Science & Technology

Tags:

• rotary
• microtome
• tissue
• cutting
• embedded
• knife
• paraffin
• coupe
• rotatie
• microtoom
• weefsel
• snijden
• histology
• histologie

[FatalMissEllen]

Wow thats so much better then I can do in school ^_^

[RONALDSCHULTE]

@FatalMissEllen

Thanks, All I can say about that is practise and practise. My first coupes where really bad but after a lot of practise it became better and better. Important is to cool the block in the fridge.

[Princisje]

Leuk dat je microtoom video's geplaatst hebt. Ik moet deze week aan de bak met coupes snijden maar ben nog een leek. De filmpjes zijn heel behulpzaam. :-)

[RONALDSCHULTE]

@Princisje Dank voor je reactie, wat doe je precies? Ben benieuwd naar jou techniek en ervaringen. Op mijn website (ronaldschulte.nl) staat nog veel meer histologie en technieken. Daar kun je me ook via mail bereiken. Groet Ronald

[UkyoKuonji2010]

Nice clean ribbon! Why can't my microtome make something like that? I end up wearing most of my ribbons or it ends up all over everything(including the microtome) XD Cooling the block doesn't help nor does changing the blade. I guess it's the kind of paraffin wax we use or something.

[RONALDSCHULTE]

Hello,

I use paraplast but use of normal paraffin is fine to. Of course is my cutting not always a party. Speed, umidity, temperature, thickness etcetera. Al those things must be perfect. When the air is to dry you can't cut one good coupe. Lung tissue gives better results than a hard Sternum. Start with 7 or 8um. That should always give a good coupe. If not than is your knife angle maybe to great or small. My angle is 32 degrees. Gr Ronald

[mesholmesho]

I always using the needle to spread the cutting tissue , but I will change to the brush because I always cutting the tissue with the needle

Thanks

[brandonjjun]

Awesome! I'm having trouble sectioning brain tissue in paraffin. I always end up with wrinkles or some kind of puckering when I mount on slides and also the sections roll over on themselves while I'm slicing. I read you suggesting refrigerating the blocks beforehand? help please!

[TheLAPosh]

@Motorcycliste ahahahha!

[AtheneWinsFan]

Fantastic!

[donehardy]

leica blades haha

[Motorcycliste]

This the machine my butcher uses at his deli. The cheap bastard cuts the pastrami this thin...