Differentiation of MM14 skeletal muscle cells.

This is a time-lapse movie of a MM14 myoblast culture induced to differentiate by depletion of serum and bFGF in the culture medium. Myoblasts exit the cell cycle and undergo fusion to form multi-nucleated myocytes. Real-time length=48 h.

Video was captured using a Zeiss Axiovert 200 with a plan-neofluar 10X objective.
Frames were acquired with a Zeiss Axiocam MRm camera using Zeiss Axiovision software (V. 4.5).

Tai, P and Hauschka, SD. Department of Biochemistry, University of Washington.

Category:

Science & Technology

Tags:

• myoblast
• myocyte
• differentiation
• muscle
• cells
• mm14
• skeletal
• mouse
• time-lapse

[ptai]

@baselta1 About 48 hours. MM14 cultures will spontaneously differentiate upon serum withdraw (1.5% horse serum). This particular culture was actually "self-conditioned"; meaning that the culture was allowed to proliferate under growth conditions (85% Ham's F10C, gentamycin, 15% horse serum and 2ng/mL bFGF) and then allowed to self-deplete local growth factors by simply not subjecting the culture to repeated medium changes.

[baselta1]

How long is this time lapse movie? In another word, how long did it take the cells to change from a single myoblasts into myotubes?

What was the differentiation (fusion) media?

Thank you