FRET - Fluorescence Resonance Energy Transfer - Dr Othon Gervasio - 3D Scientific Animation

This animation shows how FRET occurs, based on cells transfected with plasmids encoding fluorescent tagged proteins. CFP and YFP were used as donor and acceptor respectively.

Category:

Film & Animation

Tags:

• fret
• fluorescence
• resonance
• energy
• transfer
• fluorescent
• protein
• gfp
• cfp
• yfp
• interaction
• binding
• forster
• distance
• microscopy
• plasmid
• cell
• culture
• mitosis
• apoptosis

[ayanchin]

Does this mean that minimum space between proteins for direct interaction is 10 nms?

[leogervasio]

Based on the fluorescence resonance energy transfer, if the distance between the 2 fluorescent tags is less than 10nm, it is considered direct interaction. If there is another protein between the 2 proteins of interest, it is very unlikely that there will be energy transfer between the FRET pairs (e.g. CFP, YFP).

[ironnica]

@006jackass speak for yourself. Read about the basic idea of fret, ie forster transfer. one of the first things you see is the 100A max distance between molecule energy transfer

[006jackass]

why not making any audio! Not helpful at all!!

[frosaki]

This is really helpful! Thanks for uploading!

[Heybat]

باریکلا

[ulujm]

@pritesh316

Hi i do FRET and if i transfect CFP and YFP alone (with out fuse protein) or CFP alone with a fused protein to YFP or the other way around, I can't detect FRET. only when both protein are present. I never ever detected Fret with only both Fluorophore.

[laveolu]

Great video Doc, thanks. If the unknown protein(s) in-between has a cross-sectional width so small that the distance between CFP and YFP is less than 100nm, FRET?

Are you aware of any of such and what is the probablility like? Thank you.

[laveolu]

Great video Doc, thanks. If the unknown protein(s) in-between has a cross-sectional width so small that the distance between CFP and YFP is less than 100nm, FRET?

Are you aware of any of such and what is the probablility like? Thank you.

[TheArunyaj]

Great VDO !!

[pritesh316]

Hello Dr. Gervasio, Thank you for uploading such a wondeful animation.

I read FRET is nonradiative energy transfer ... what does tht mean ??

Also, FRET depends on proximity of donor and acceptor fluorophores ... so its not necessary that protein interatcion should occur to detect FRET. It can also happen if two fluorophores come in close contact without actual interaction b/n protein. How can you justify that ?

I would appreciate if you reply.

[geyserli]

So we can know under which condition the unknown protein goes away? That's why we do this experiment? Why don't the two fusion proteins meet from another direction to avoid the unknown protein?