BioFlux System for Live Cell Assays Under Controlled Shear Flow (Fluxion Biosciences)

BioFlux provides a fully integrated solution for running biologically relevant cellular assays under shear flow. The system utilizes Fluxion's Well Plate Microfluidics™ (WPM) to provide high content results from a convenient well-plate format. Precise control over shear flow enables a wide variety of cellular and microbiology applications which call for vascular emulation, mechanical stress, compound/buffer exchange and high content analysis.

BioFlux enables a wide variety of cell-based assays in the fields of immunology, vascular biology, microbiology, cancer research, stem cells, and more. Many of these applications benefit from the ability to create a physiologically-relevant shear flow profile, such as platelet adhesion, cell adhesion, rolling velocity, transmigration and stem cell differentiation. Other applications can benefit from the ability to introduce compounds in a time-resolved and well-controlled manner, such as wound healing, pharmacology and neurite-outgrowth assays.

Category:

Science & Technology

Tags:

• bioflux
• live
• cell
• assay
• imaging
• adhesion
• platelet
• wound
• healing
• biofilm
• stem
• cells
• microfluidic
• invasion
• migration
• angiogenesis

[FluxionBiosciences]

@numbaslevin

That's actually the nice part about the system...the fluid doesn't run through those lines. Those tubes are only for air pressure, which drives the flow of media, cells, etc, from the well into the microfluidic channels. So as long as you heat the well plate (see previous response), you're entire experiment including reserve fluids will be kept at the same temp.

This also helps with clean up....those tubes never have to be cleaned since it is only sterile air going though them.

[FluxionBiosciences]

@numbaslevin

Yes, we have a few different heating options depending on your scope and application requirements. We have an integrated heater plate for most of our systems, as well as full environmental enclosures for more controlled applications.

Using our pneumatic approach, air bubbles are avoided. We have a simple priming step which fills the channels, then they're free from bubbles for the duration of the experiment. We still recommend the basic precautions such as degassing solutions.