Micropropagation Of Venus Fly Trap Part 2 (Tissue Extraction And Sterilization)

2nd part of my homemade video!　This time for the tissue culture of Venus Fly Trap.
For those interested. this is the link to the photo album i have online for the cloned traps
http://www.flickr.com/photos/27449192@N04/sets/72157626134543886/with/5476519...

Category:

Education

Tags:

• Venus
• Fly
• Trap
• Tissue
• Culture
• Micropropagation

[flytraplady]

Thank you, I am a venusflytrap nursery propagat looking into learing the cloning process, your video is helpful, but I everytime I watch it Im looking at your equipment, being a novice I recognize little, if possible could you post suggested equipment list, it would save me ALOT of time in research, ")

[swatpup6433]

@flytraplady if you are planning to start from scratch, equipments i would recommand include a weighing scale, a electronic PH meter, not sure if you could find an autoclave, but a pressure cooker would work too. and a clean "glove box" to work in. magnetic stirrer and heating plate is a luxury item IMO. u could work by manually heating and stirring the mixture.

[skateboy159]

is there a way to make medium from house hold stuff if so can u tell me how to make it

[swatpup6433]

@skateboy159 u could use household appliances for these. however stuff like MS media. its quite hard to formulate you could buy them in pre mix packets thou. try this website.

hometissueculture dot o r g

[pquoc64]

at 3:11 what is the liquid in the jar??Javelle water??

Thank you for the video, its very informative.

[swatpup6433]

@pquoc64 hi, the liquid that the cuttings were transferred into at 3:11 is a diluted bleach solution. 9 parts distilled water to 1 part bleach.

[TheCarnivoras]

con este metodo se pueden multiplicar las venus pero tiene que ser en su etapa adulta creo

[jofly17]

Great job.

[swatpup6433]

Glad you like it. hopefully I could make one on the division and transfer of VFT from agar medium to new agar medium if this batch starts growing successfully. I was actually waiting for your video on that! lol from your last vft video you said something about cutting up the small one. will be nice to see how the transfer is done on video. It waS from your video that i learned that a cutting of the leaf would work. normally i would dig up the plant and cut a tissue off the rizome area.