Plant Tissue Culture Media Preparation

PhytoTechnology Laboratories presents Media Preparation, an informative video highlighting the steps to prepare plant tissue culture and micropropagation media from powdered formulations.

For plant tissue culture media, supplies, equipment, biochemicals, antibiotics, and plant growth regulators, please visit our website at www.phytotechlab.com. You can also become a fan of PhytoTech Labs on facebook to keep informed of new product introductions, recent laboratory studies, and other news.

Category:

Science & Technology

Tags:

• phytotech labs
• plant tc media
• powdered formulations
• agar
• gelling agents
• autoclave
• pressure cooker
• culture vessels

[Gemthermakleingk]

Veri nice video. I see before autoclaving, the girl is closing the tissue culture jars and the caps are not placed completely, i mean some sides are still up. is it done intentionally? i want to use baby food jars. should i place and close them tightly or should i just put the cap withought tighten it before autoclaving? Thanks phytotechlabs.

[PhytoTechLabs]

@Gemthermakleingk Yes, this is done intentionally with polypropylene containers like these. The lids seal tightly which results in deformed containers when the pressure drops at the end of the autoclave cycle. This is circumvented by leaving the lids slightly ajar and then closing them when the autoclave is opened at the end of the cycle. This is not a problem with baby food jars using our PhytaCaps or Magenta B-Caps.

[340070007]

autoclaving the media including plant growth regulators causes hormon deformation.

[PhytoTechLabs]

@340070007

Most plant growth regulators are routinely autoclaved in the medium, especially the stable ones such as BA & NAA. Some are heat labile and should be filter sterilized and added after the medium is autoclaved and cooled a while. Our "Plant Growth Regulators, Part II" document on the Technical Info page on our web site offers guidelines for whether the plant growth regulator can be autoclaved in the medium or filter sterilized.

Gary Seckinger

Technical Services

PhytoTechnology Labs

[Rannyfash]

you should be carefull when adding microwaved water to another medium as it can be superheated with no pitts to change state in the smooth glass flask

[athulabar]

Excellent presentation.... ..i suggest you to prepare more video clips like this to educate the people those who work with this field...

Thank You Very Much.

Bimal....

[goldslinger]

Thank You so much for Your continued effort with these videos!

Please keep them coming.

Gary

Garden City, KS