Ph.Diddy is on the scene - Animation of life in the biotech lab

The new sequel is here! (Ph.Diddy at the conference) http://youtu.be/ajdbuj8P784
and also see (Ph.Diva) http://www.youtube.com/watch?v=ryhSGU... PCR

Arnold Young (aka the Ph.Diddy) is a biotech Ph.D candidate. Arnold was an A+ student, and is now ready to take over the world of science! He soon discovers how life in the lab is filled with drive and devotion, frustrations and fulfillment, hard work, late hours, repeated experiments, peer review and a strive for respect and recognition. How will the ups and downs of life in the lab shape our Ph.Diddy on his journey to have his first scientific paper published?

Acknowledgements: This video is inspired by all the dedicated everyday-heroes-of-science. Invitrogen recognizes your passion and admires your perseverance!

BY POPULAR DEMAND - the song is now available on iTunes!
http://itunes.apple.com/us/album/the-...


--------------------

Lyrics:

I was the black belt of my class, marked out as an achiever. First day on the job, enter the gene-weaver.
My future colleagues look up from the PCR machine, checking me out man. The Ph.Diddy's on the scene.
So I claim my pipette and pimp it up to halt the theivin'. Get the thermocycler going, my samples cool chilling.
Yeah, I shoot tips like I've been here all my life. Waiting for instrument time with my free pizza slice.
I run my first Western blot. Man, this lab scene is easy. But wait up, hold a minute. I'm feeling kinda queasy.
Is that Trizol I can smell? Oh, my head's in a spin! 've gone and dropped my freakin' sample in the biohaz bin.

He's a super fly grad. Life plans to shape the future, that's right!
Sees his name in lights and a centre spread in Nature. All right!

I'm always splitting cells, always working late. I've cleared the biohazard bin so much I herniate.
Got samples botched, samples lost, samples dropped on the floor. Messy benches, empty buffers, lab politics galore.
My PI's buggin'. Man he sounds like my father. Western blot once again, but development shows nada.
I'm doin' my time in the cell culture hood - at the weekend bro. It's the only time I could!
Then suddenly my data is starting to make sense. Even Western blot bands start to make an appearance.
So I repeat once again all the steps I need to follow. But every culture well... turned yellow.

He's a super fly grad. Life plans to shape the future, that's right!
Sees his name in lights and a centre spread in Nature. All right!
I'm talkin' 'bout the ooh, ooh...

Serum and media get swiped with no apology. And all my cells are showing abnormal morphology.
So now my PI's freakin' "Find something you can prove!" But contamination aggravation knocks me off my groove.
So I stay late once again as the lab detainee. Yeah, its 9 pm but the thermocycler's free
I don't understand! What's happened now? That's it. Yeah, time to give up. I'm throwing in the towel.

Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah!
Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah!

My transfection worked - confirmed with Pol II chIP - mutagenesis determined the TF targeted.
I'm not going down! I can still do BioChem! My genes will express, I'm gonna beat it out of them.
And yeah, I must have results that no one can undress. And quickly move my paper from in prep to in press.
I'm finally finished writing the discussion, whilst my PI once again, "tweaks" my introduction.
So, here we go, yo. Here's the scenario: Peer review's due to hit. When exactly? I don't know.
My paper will be published. My research is legit. OK it's not quite Nature but, I think I nailed it!

He's a super fly grad. Life plans to shape the future, that's right!
Sees his name in lights and a centre spread in Nature. All right!
He's a super fly grad. Life plans to shape the future, that's right!
Sees his name in lights and a centre spread in Nature. All right!
Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah!

• Category

  Science & Technology

[Chaitanya Dadhich]

Now, I'm not trying to be racially oversensitive or anything but isn't the Indian girl's skin tone a rather ghastly shade of green? I mean, I do agree we are *brown* but in all the ancient scriptures there is no mention of our ancestors mating with martians. Its an eyesore, that dirty green, not racially but from a design viewpoint. It stands out in a very non-pleasing way.

Other than that, loved the video! Ooh Ooh, Supergrad! :D

[LifeTechnologiesCorp]

Totally agree Chaitanya - we picked up on this design error and changed it in the sequel: "Ph.Diva and the Mystery Band"

Glad to hear you like the video :)

You'll find links to the sequels in the description.

[LifeTechnologiesCorp]

@stein haynes Stay tuned, we're adding that final touch to the third video in this series now. Will be launched in a week or so!

[LifeTechnologiesCorp]

The third video is up, you'll find the link in the description. Enjoy!

[rhinolola]

I love this. I don't have the PhD but I went through the process for my Masters. Does anyone know what PI stands for?

[LifeTechnologiesCorp]

The PI is the 'Principal Investigator' - the lead scientist and research lab group leader.

[LifeTechnologiesCorp]

Average salary will vary, as there are many different job titles within the field. It would also depend on where you live, and the type of institution. Try Google!

Not many start a career in science to get rich :) The motivation for pursuing such a career typically comes from a wonder and fascination for science, and a certain level of curiosity. Most scientists do science because they want to do research. They are passionate people!

[LifeTechnologiesCorp]

This is how it goes:

Chorus:

Cm, Eb, F, Cm

Cm, Eb, F, Cm

Verse:

Cm, Bb, Eb, F

Cm, Bb, Eb, F

Cm, Bb, Eb, F

Cm, Bb, G7 G7

[Partha Doshi]

The best one among the 3 ...feels proud being Biotech engineer.. :p

[echrobok audrea]

Your video is awesome.

[helfrich raven]

I'm loving this movie.

[yvanhorn dorine]

this is so cool.

[nbucholt karyl]

Great video. So cool.

[SuperEndospore]

Seriously if Life technologies sold Ph Diddy t shirts Id buy one. That would be awesome.

[Paul Plante]

Liked and shared.

[Sheriman Af]

Liked and shared.