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High Performance Liquid Chromatography HPLC

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Uploaded by on Sep 27, 2008

A chemistry education video from the Royal Society of Chemistry on High Performance Liquid Chromatography (HPLC) included on the "Modern Instrumental Techniques for schools and colleges" DVD. For more information on the Chemistry for our Future programme please visit http://www.rsc.org/CFOF

(C) Royal Society of Chemistry

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  • What is the price of a unit like this?

  • Please email library@rsc.org and they will help you with your enquiry

Top Comments

  • Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,

  • brilliant.

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All Comments (49)

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  • @282sanjay

    Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract

  • British people are good at explaining things.

  • wonderful! 

  • is their any method for the analysis of thyroid hormone like T3, T4, TSH and calcitonin using HPLC Technic. if yes then please help me by giving the prepareation and appropiate column for the analysis of the above from serum sample

  • bravo bravo score

  • I'm amazed at what software has done for these things over the years. Anymore you almost never need to touch the controls on the components. This new controller we got in our lab just lets us set up our methods, standards, unknowns, and whatever else and then runs the batch with more samples than you would ever care to run. I can even have it run a shut down method to flush the column, cool the oven, shut the lamp off, etc. It'll do everything short of having your coffee ready in the morning

  • @saurabhCherished I would suggest normal chromatographic columns, if the product is well known, an affinity column might be aqble to do the job.

  • What model of HPLC is this? thank you.

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