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Chemotaxis of dendritic cells

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Uploaded by on Jan 12, 2007

SKBR-5 ist eine menschliche Brustkrebs-Zelllinie, die wie viele epitheliale Tumore Lewis-Y positiv ist. SDZ ABL 364 ist ein monoklonaler Maus anti Human Lewis-Y Antikörper. Heparinisiertes Blutserum, welches ein aktives Complementsystem enthält, setzt den chemotaktischen Lockstoff C3a am antigengebundenen Antikörper frei. Die einzigen eigenbeweglichen Zellen sind die dendritischen Zellen. In Echtzeit würde dieser Film etwa 50 Minuten dauern.

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  • The antigen is located on the outside of the cell membrane.

    The antigen is not optical visible.

    The granules inside the cells are cell organells.

    The granules outside the cells are thrombocytes.

    The chemoattractant is C3a from activatet complement system.

    C3a is a soluble protein, C3a is not optical visible.

    No red blood cells (RBC) are in this video.

  • wow, that was amazing. couple questions though:

    1. after phagocytosing the "antigen," were those granules that it spit out?

    I assume that because it lysed the cell (RBC im guessing by it's shape) right next to it.

    2. is it those granules that work as chemoattractants??

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