Cell Isolation - The Two Worlds of Cell Separation

Cell isolation, See REFERENCES below. An entertaining and useful explanation of the differences between the micro- and nano-particle techniques used for cell isolation / cell separation.

Articles from RICE (mentioned in video): http://bit.ly/gPytFD, http://bit.ly/gXo3KY, http://bit.ly/i5i8kI, http://bit.ly/ergkVa

Article by Dr. Shenoy (mentioned in video): http://bit.ly/dLZPqf

Article on cellular responses to dextran-derivatised iron oxide nanoparticles: http://bit.ly/i4BPbQ

Article on uptake of dextran-coated nanoparticles: http://bit.ly/fQEU5d

Article indicating induction of forced cellular iron elimination of nanoparticles: http://bit.ly/i72Nnd

Article on iron oxide nanoparticle toxicity: http://bit.ly/gUTAeu

Article on polystyrene nanosphere toxicity: http://bit.ly/heJWJp

Article on nanoparticle impacts on gene expression: http://bit.ly/fQ58wx

Article on cell response to dextran-derivatised iron oxide nanoparticles post internalization: http://bit.ly/hbhWnH

Article on dextran derivatised iron oxide nanoparticles and their influence on fibroblasts: http://bit.ly/dW1CCy

Article on nanoparticle cytotoxicity and changes in cytokine induction: http://bit.ly/dFdifn

Article on immunotoxicity of nanoparticles: http://bit.ly/dFGkyX and http://bit.ly/ggkhjr

Article on metal oxide nanoparticle toxicity: http://bit.ly/fphwwh

Article on receptor mediated endocytosis of nanoparticles: http://bit.ly/hMk7vM

From the fields of nanomedicine and nanotoxicology: http://bit.ly/gpMsA7, http://bit.ly/cDOxDn, http://bit.ly/gv71Wp, http://bit.ly/frF180, http://bit.ly/fTJTf8, http://bit.ly/17wHLO

Journal dedicated to nanotoxicology: http://informahealthcare.com/nan

More refs: http://en.wikipedia.org/wiki/Nanoparticles#Safety

The European Commision's initiative on the potential harmful effects of nanoparticles: http://bit.ly/g4gnUo

What level of uncertainty is acceptable? When searching the literature, we find plenty of articles where the use of nanoparticles seem to have no negative effects in a variety of settings. Along with plenty of articles demonstrating the opposite - that nanoparticles do indeed have a detrimental impact.

When will the knowledge and concerns about nanoparticles from the field of toxicology bridge over to the continent of cell isolation?

Category:

Science & Technology

Tags:

• Immunology
• Cell separation
• cell isolation
• cells
• T cell
• CD4
• CD8
• CD3
• mouse cells
• human cells
• Miltenyi
• Dynal
• Invitrogen
• dynabeads
• flow cytometry
• columns
• column-based
• magnetic beads
• magnetic particles
• microbeads
• nanoparticles
• dextran
• ficoll
• rsa
• animation
• cartoons
• information graphics
• cognitive media

[jlmknight]

is that the same hand that does RSAnimation?

[cellisolation]

@jlmknight Correct. Don't you just love their work?!

[gdoitsh]

I am not convinced that microbeads create higher magnetic power. Many nanobeads can cover a single cell, providing an equal magnetic force. Also, because of their small size you can use them to isolate about 400 millions cells on one column.

In addition, resting cells (like blood lymphocytes) do not support endocytosis and therefore do not internalize the nanobeads (unless you pre activate them).

Overall that was a very informative presentation. Good job!

[cellisolation]

@gdoitsh Glad you like it!

Capacity is not related to bead size. When you cover each cell with thousands of nanoparticles, you still need to use a column. The column contains big magnetic particles, amplifying the magnetic field by 10,000-fold to enable separation.

What the potential effect is of nanoparticles accumulating inside and/or on the cell surface is not yet fully understood.

Feel free to send us an email at ‘eurotech@lifetech.com’. Would love to discuss this in more detail :)

[ColetteLnA]

This is definitely the craziest but most creative video I've ever seen about Cell Isolation.

[loganmcafeetv]

why am i watching this. i dont even know how i got here.

[93tomb]

@gdoitsh If each bead covered an amount of surface area proportional to the mass raised to the power of 3/2, wouldn't larger beads result in more force anyway? I dont know much about the way they attatch to the cell though, you may be right.

[93tomb]

You've lead to this Physics undergraduate learning a bit more about cell biology. Thanks :)

[Olaoo99]

Didn't understand too :S

[TheSovietOverlord]

@TheSovietOverlord The illustration is good, i just don't understand.

[TheSovietOverlord]

Why is this important?

[gadionson1]

i have no idea.. !!

[ben3customhomie]

i did not understand a thing i came here from terraria