DNA Lab Part 2

Purchase: http://hilaroad.com/video
This video includes step by step instructions for extracting DNA from wheat. This procedure can easily be done at home or school with readily available equipment and supplies.

Category:

Science & Technology

Tags:

• DNA
• extraction
• PCR
• genetics
• lab
• wheat
• Einkorn
• neolithic
• iceman
• science
• fair

[alphadog7473]

that is a great video , many thanks !

[beeecraft]

I believe that the positive charged Na ions bind to the negatively charged phosphate backbone of the DNA molecule, causing an opening of the secondary (and tertiary) structure. The atoms force open the molecule through steric hindrance, similar as to why all molecules have defined angles and shape. Also, they prevent the binding of positively charged histone proteins that help wind the DNA.

[xtaxinyoface]

Its the same effect as oil on water

Oil is on top of the water.

DNA is the oil

Water is the alcohol layer

[nlgatewood]

this is a little late, lol..  But the DNA seperated out because it does no disolve in the alcohol like the other stuff does. It's like oil and water. Oil does not dissolve in water and floats on top.

[pushp01]

Such a nice video i ever watched!!

[SAJJJJJAD]

AWSUM....!

[dreamy2]

Why did the DNA entered the alcohol layer????

[prayfertrey]

I wonder if there is any pectin in that.

[DawnFoxworth]

Wonderful!

[givemelols]

what happens if I try to look at those dna strings in a powerful ordenairy microscope? :P Will I see anything that look like a dna or just a thin layer of fibers?

[interstitialofficial]

So, when dna is precipitated in alcohol, was it dissolved, or was it in it's original molecularly thin form, just not clumped with the other strands? Many thanks.

[Melkor10]

DNA can be dissolved, it can be denatured into its constituent thingymagics (phosphate, base, deoxyribose sugar). Denaturation can occur from high temperature, high concentration alkaline solution, high concentration urea and high concentration of formamide