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How to Make Fluorescein (Fluorescent Dye)

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Published on Oct 13, 2012

In this video we show how to make fluorescein, a strongly fluorescent chemical.

Combine 166 mg of phthalic anhydride, 220mg of resorcinol and 3 drops of concentrated sulfuric acid. Heat to 200 degrees celsius for 5 minutes. The crude fluorescein produced is dissolved in dilute sodium hydroxide solution (300mg in 10mL of water). To demonstrate fluorescence a few drops of the solution are dropped into water under ultraviolet light.

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