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widal test (slide & tube) procedure

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Uploaded by on Mar 28, 2011

Widal test is still a common way of diagnosing enteric fever cases in several countries. Although tube agglutination is the standard, some manufacturers have adapted it for slide agglutination. This video shows qualitative as well as quantitative test on a kit that can be used for both slide as well as tube agglutination. Procedure displayed here is as per manufacturer's directions. Procedure of tube agglutination test varies across kits. Kits designed exclusively for tube agglutination have different procedures.

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Uploader Comments (microrao)

  • In serial doubling dilutions, certain volume from the preceding tube is mixed and transferred to the subsequent tube. In doing so, the volume becomes more than preceding tube, therefore the volume received must be lost as well.

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  • Thank you :) this video helped me a lot..

  • Excellent video, and well needed-Wikipedia dropped the ball where the Widal test is concerned. Question: Why was 1 millilitre discarded from the last tube?

  • make more of such good videos

  • well demostrated great

  • great video!!! many thanks!! :))

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