Passaging Cells: GIBCO® Cell Culture Basics

The growth of cells in culture proceeds from the lag phase following seeding to the log phase, where the cells proliferate exponentially. When the cells in adherent cultures occupy all the available substrate and have no room left for expansion, or when the cells in suspension cultures exceed the capacity of the medium to support further growth, cell proliferation is greatly reduced or ceases entirely (see Figure 4.1 below). To keep them at an optimal density for continued growth and to stimulate further proliferation, the culture has to divided and fresh medium supplied. This video explains why, when and how to passage cells grown in both adherent and suspension cultures.

The entire Cell Culture Basics training course is available online at http://www.invitrogen.com/cellculturebasics. The handbook and videos provide an introduction to cell culture, with a focus on maintaining cell health throughout the processes of culturing, freezing, thawing and passaging cells. The handbook also contains troubleshooting guides and protocols for passaging, counting, freezing and thawing cells.

Category:

Science & Technology

Tags:

• cell passaging
• passaging cells
• Cell culture split
• subculture
• cell detachment
• cell dilution

[IcySkeptic]

Thank you :-). This video is excellent review/tutorial!

[minnyo1991]

what is complete growth medium ????