Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
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@BigBoss7777777
unsure bigboss, I've not very familar with PRP
squidy1000 4 months ago
@squidy1000 Does this work for creating Platelet Rich Plasma aswell?
BigBoss7777777 4 months ago
I realized I made 5L's of PBS using a 1L recipe, thus my cells were being lysed since my PBS wasn't isotonic. Thank you for your advice though.
ihatefinishline 1 year ago
@ihatefinishline
spin at 800g and it will work fine, it won't make a difference to cells. I spin at 800g (for 20 min, brake off) all the time and i have have done 1000's of seperations.
400g doesn't work for me when i have tried it. Most labs i know use 700-800g.
squidy1000 1 year ago
I followed this exact procedure, and for some reason failed to obtain a gradient and separation. Do you have any ideas as to why this occurred?
ihatefinishline 1 year ago
Neat Video
chaochavez 2 years ago