DNA Sequencing

DNA Sequencer

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Howto & Style

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• bio1

[IloveYOUviruses]

WHAT!? DNA in a conveyor belt????? AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA­AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA­AAAAAAAAAAAAAAAAAAAA(must be telekinesis) -_-

[befz88]

ya okay, but it doesnt explain the whole sequencing process.

[19Tranc3r92]

Oh wow, go laser/photons!

[viking488]

It's just Sanger sequencing but using fluorescently labelled ddNTPs, one for each colour. The only difference is you don't need four lanes on the gel

[gayasadaisy]

is this automated sequencing or what? and why do the special dideoxy-bases fluoresce different colours with the laser? do scientists add something special to them to make them coloured? or is that just the natural way they're coloured? gah, I need more details D:

[Dava1717]

Can anyone explain the Maxam-Gilbert Method?

[marcianos]

Up to what I know (and as you can see in the video), you can run all of them in the same tube as long as each ddNtP has a different fluorescent mark. If They all have the same mark, then you need to run them in different tubes.

[bruckwine1978]

ya mark just like that 4 separate tubes for each base to read one sequence :)

[bruckwine1978]

Yea that's right mark - so they need to run all 4 rxns in 4 separate tubes to read one sequence just like that.

[markgg1]

Thanks!

its just clicked in my mind how it works I think

E.g. for length 1 bases, the end might be A

2 bases would end T

3 bases end C

4 bases end G

So, it would read ATCG?

Am I right?

[adnansaami]

does anyone have a flow diagran of how to sequence dna using whole blood using the sanger method