This video is a collaboration between plant cell biologists at Oxford Brookes University, the University of Warwick and the musician Cyrus Mower. We love plant organelles and we want to share our passion with the world!
@PandasPet Thanks for your comment! I am copying in the reply from Dr Lorenzo Frigerio:
Unless you double-label with FM4-64 it's very difficult to tell plasmamembrane and tonoplast apart. In this case the telltale signs are the little transvacuolar strands that seem to protrude into the vacuolar void (there's a nice one at the center top, roughly before the 'g' of largest).
great song! at 0:22, though, it looks like the image shown is actually of GFP fluorescing in the plasma membrane or cell wall - those jigsaw puzzle-like shapes are the cells on a leaf surface (you can even see some stomata in there) and if the fluorescence is in the tonoplast, then these cells appear to be literally ALL vacuole!
@PandasPet Glad we could help!
PS: Vacuoles are going to dominate the world (together with Golgi bodies, of course).
plantendomembrane 1 week ago
@plantendomembrane cool! i had no idea that the vacuoles were so dominant in the pavement cells. i was hoping someone in the know would respond!
PandasPet 1 week ago
@PandasPet Thanks for your comment! I am copying in the reply from Dr Lorenzo Frigerio:
Unless you double-label with FM4-64 it's very difficult to tell plasmamembrane and tonoplast apart. In this case the telltale signs are the little transvacuolar strands that seem to protrude into the vacuolar void (there's a nice one at the center top, roughly before the 'g' of largest).
plantendomembrane 1 week ago
great song! at 0:22, though, it looks like the image shown is actually of GFP fluorescing in the plasma membrane or cell wall - those jigsaw puzzle-like shapes are the cells on a leaf surface (you can even see some stomata in there) and if the fluorescence is in the tonoplast, then these cells appear to be literally ALL vacuole!
PandasPet 1 week ago