Less Time to Real-Time PCR with Ambion® Cells-to-CT™ kits

http://www.lifetechnologies.com/cellstoct
Gene expression analysis is so much easier when you can leave out the mRNA isolation step altogether, and instead prepare your cells directly for real-time PCR.

You'll get your real-time PCR results in less time with the Ambion® Cells-to-CT™ Kits. It is fast, easy and robust. Fewer handling steps ensure shorter protocol time, increased consistency, minimal sample loss and a reduced risk for contamination.

Learn more at http://www.lifetechnologies.com/cellstoct

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[audio transcript]

Traditionally, the first step in real-time PCR is to isolate the RNA. This isolation step can require significant time and involve multiple handling steps, and a risk of sample loss. Ambion® Cells-to-CT™ Kits provide a simple and rapid method for preparing cell lysates. Relative gene expression is measured directly from cell lysates, eliminating the need to purify RNA prior to amplification.

Ambion® Cells-to-CT™ Kits use the simple 7-minute sample preparation procedure. First ten, to a hundred thousand cultured cells are washed with phosphate-buffered saline. They are then mixed with Lysis Solution, and incubated at room temperature for 5 minutes. The Ambion® Cells-to-CT™ also reduces the risk of contamination and loss of sample since there are few steps in which the sample is pipette. Cells are lysed during this incubation and RNA is released into the Lysis Solution which also contains reagents to inactivate endogenous RNases. If DNase I is added to the Lysis Solution, genomic DNA is also degraded at this step. 5 minutes later, a Stop Solution is mixed into the lysate to inactivate the lysis reagents - leaving you with a sample, ready for Real Time PCR with results equivalent to pure RNA. No heating, washing, or centrifugation steps are required as are for traditional RNA purification methods.

Category:

Science & Technology

Tags:

• qPCR
• PCR
• real time
• real-time
• RT PCR
• qRT PCR
• qRT-PCR
• cells
• to
• ct
• cells to ct
• ambion
• taqman
• 2 Step
• mRNA isolation
• mRNA
• mRNA purification
• RNA purification
• reverse transcription
• gene expression analysis
• master mix
• life technologies
• rtpcr
• RT-PCR1
• Single cell
• Single cell analysis