Protein Identification by MALDI-TOF

Matrix-assisted laser desorption/ionization-Time Of Flight (MALDI-TOF) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is most similar in character to electrospray ionization both in relative softness and the ions produced (although it causes many fewer multiply charged ions).

The ionization is triggered by a laser beam (normally a nitrogen laser). A matrix is used to protect the biomolecule from being destroyed by direct laser beam and to facilitate vaporization and ionization.

Category:

Education

Tags:

• MALDI-TOF
• MALDI MS
• Protein Identification
• Differentially
• expressed
• protein
• Selvam
• TNAU Genomics lab
• TNAUgenomics

[startimes7]

this is a good clip but plaese add subtitles because i dont understand a word of what you're saying...it sounds indian

[prifun]

This is not just indian......Specifically SOUTH INDIAN accent...Even Other Indian people don't understand their accent!!!

[5257gina]

terrible...terrible accent. I don't understand a single word

[mr0123456789122]

@startimes7 dis is south indian accent

[TheOriginalChinaMan]

So Apu does more than just own Kwik E Mart

[romeolanddy]

My friends indian and he couldnt even understand that says alot sort it out boy!! awfull !! didnt understand 1 bit of it .. all i got was time of flight :S

[MsStudytime]

PLEASE add subtitles to this video. I couldn't learn what you were saying because I couldn't always deduce what you were saying.

[Acks2000]

sort your sound out man!