Glowing Physarum

Glowing Physarum: First, a piece of Physarum plasmodium is homogenised with a drop of (concentrated) fluorescent particle solution on a glass slide. Then they are transferred to a 1.5% agar gel and cultured overnight. This part of physarum in the pictures is a small branch growing from the mixed physarum/fluorescent particle culture. Thus the particles are *in* the physarum cell, not on the slime layer. In fact some particles are flowing along with the protoplasmic streaming.

Fluorescent particles used: FluoSpheres carboxylate-modified microspheres (Molecular Probes, USA). 0.5um, yellow-green fluorescent (505/515)
Filter used: FITC filter (for detection of fluorescein isothiocyanate, which wavelength is approximately 495 nm/521 nm)

Category:

Science & Technology

Tags:

• physarum
• fluorescent particle