Sequencing DNA RNA Nucleics Acids Secuenciacion ADN acidos nucleicos

DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. Thus far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates. However, new sequencing technologies such as Pyrosequencing are gaining an increasing share of the sequencing market. More genome data is now being produced by pyrosequencing than Sanger DNA sequencing. Pyrosequencing has enabled rapid genome sequencing. Bacterial genomes can be sequenced in a single run with several X coverage with this technique. This technique was also used to sequence the genome of James Watson recently.

The sequence of DNA encodes the necessary information for living things to survive and reproduce. Determining the sequence is therefore useful in 'pure' research into why and how organisms live, as well as in applied subjects. Because of the key nature of DNA to living things, knowledge of DNA sequence may come in useful in practically any biological research. For example, in medicine it can be used to identify, diagnose and potentially develop treatments for genetic diseases. Similarly, research into pathogens may lead to treatments for contagious diseases. Biotechnology is a burgeoning discipline, with the potential for many useful products and services

Category:

Science & Technology

Tags:

• Sequencing
• DNA
• RNA
• Sanger
• Gilbert
• Big
• dyes
• terminators
• molecular
• biology
• genomics.
• Secuenciación
• ADN
• ácidos
• nucleicos

[happinesson]

wow! the complex DNA world..nice video!

[adelle0001]

nice! makarember ko sa aqng class way back in high school.

[arunmoy]

Excellent video. I have a question. How can it be assured that DNA extension is stopped at each and every base in some or the other strand? ( I mean, can the ddNTPs can "miss" some bases enroute the chain?)

[fablesergio]

I think that depends, maybe, on the amount of dNTPs and ddNTPs or maybe I missunderstood you question.

[bonovianna]

that's some veeeeery nice video... I've been looking for a sequencing explanation on youtube for a long time! Is there other methodologies for Nucleic Acids sequencing?

[bonovianna]

Large fragments would probably be scarce but they would be detectable anyway... So, I guess this wouldn't be a problem.

[marcianos]

I don't know if i'm getting this right... Isn't it highly improbable for the polymerase to place hundreds of dNTPs before adding one ddNTP? wouldn't large fragment be scarce?