Antibody Purification (Protein A Column)

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Uploaded by on Apr 6, 2010

( http://www.abnova.com ) - Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid. More videos at Abnova http://www.abnova.com

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