Isotonic Solution, Hypertonic Solution, Hypotonic Solution.

Tonicity experiment : Red Blood Cells subjected to Isotonic, Hypertonic and Hypotonic solutions. Video captured at x400 magnification. Hypotonicity video is of the highest quality I have seen as a perfect salinity was found after many trials to produce a slow, real time haemolysis.

Many thanks to our resident IT expert and lecturer at ACU National: Majella Hales for her expert software/editing assistance.

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Education

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• TONICITY

[Mags9229]

So in the Hypotonic solution, the cells actually burst. We had a lab today looking at this very same thing except we did not get to see the process we had to guess which one was hypo hyper or iso. On one slide it looked like pieces of the membrane were scattered everywhere, or it was extremely extremely shriveled into tiny fragments. I could not tell if this was Hypo or Hyper. What would you say it was?

[superunknowner]

Its hard to know without seeing it myself. Was there a stain used on the slide? If not, membrane debris is difficult to see and it would most likely be a hypertonic solution that crenated the cells into fragment looking clumps of membrane.

[ajaykeer23939]

When RBC are in a Hypertonic, Hypotonic, and isotonic, how do you tell which one is clear or cloudy under the microscope??

[superunknowner]

@ajaykeer23939

You cant tell if the solution is hypo, hyper or iso by looking at how cloudy it is. You can tell what solution it is by effects on the RBC membrane.Haemolysis, crenation or no change respectively.

[kapoyyyyzzz]

does the isotonic is from lower solute concentretion to a higher solute concentration? what will happend to the external cell? will it rupture or sink?

[superunknowner]

Isotonic means that the salt concentration of the extracellular fluid equals the concentration of the cells intracellular fluid. Cell morphology does not change.

[mackblack22]

I agree this is a great visual example. Thanks for the reference to crenation & thanks for sharing! This is something I would otherwise not get a chance to see. I'm sure students would appreciate the reference.

[crazyghetto978818]

this video is sooo exciting!! omfg this is the best example!..

[mjXplains]

Wow, Bubbles.

[xAbercrombieFitchx]

Oh thought my speakers had broke.... Just no sound eh .

[lastheat32]

theyre vanishing

[TNTlovesmusic2011]

Thanks for sharing! :) It helped me understand my biology lab better!

[PEACEGIRL171]

WHT THE HELL IS IT!!!!!!!!!!!!!!!!!!!

[Mags9229]

@superunknowner No, there was no stain on it. It was very confusing, our teacher would not give us a def answer. The slide barely contained anything on it, it was white for the most part except maybe 3 tiny tiny tiny pieces of the membrane. In a Hypo solution do the cells actually disappear themselves?