1 7mmAutoSampler 20090918

The high throughput enabled cryo-microprobe is an essential structural proteomics tool. The screening of buffer conditions and construct optimization has historically been a laborious and materially intensive process. For instance, set up and data collection on a conventional 5 mm probe requires a minimum 20-minute time commitment and ~10-20 times the material required of a 1.7 mm cryo-microprobe. By extension, one hundred samples would necessitate a 33-man-hour commitment. The NESG has reduced the data collection time period by 75% and eliminated manual sample changing by employing a Bruker automatic sample changer and 600 MHz spectrometer equipped with a 1.7 mm micro-cryoprobe (with automatic tuning and matching). Now nearly a hundred samples can be screened in one day.

The cryo-microprobe confers a 14-fold gain in sensitivity over the standard 5 mm probe. An increase in mass sensitivity is conferred by the miniaturization of the receiver coil and a signal to noise enhancement is the result of cryogenic cooling of the electronics. Routinely performed are NMR analyses of protein samples at volumes as small as 35 mL and at concentrations as low as 200 mM. Cryo-microprobe technology has gone beyond screening and is now utilized for mass-sensitive applications (Aramini et al., 2007), backbone and side-chain assignments and to generate NOESY constraints for structure calculations (PDB ID: 2klb).

Category:

Science & Technology

Tags:

• NESG
• PSI
• NMR
• SGKB
• cryo-microprobe