Matchmaker™ Gold Yeast Two-Hybrid System: The Principle

For more information, visit: http://www.clontech.com/US/Products/Protein_Interactions_and_Profiling/Yeast_...

-Yeast two-hybrid systems exploit the modular nature of eukaryotic transcription factors (e.g. GAL4) which consist of two separable domains, a sequence-specific DNA-binding domain (DNA-BD) and a transcription activation domain (AD).
-Your known protein of interest is fused to the DNA-BD to create the bait protein.
-Interacting partner (library) proteins are expressed as fusions to the AD, creating prey proteins.
-If a prey protein interacts with your bait protein in the same cell, the DNA-BD and AD are reunited, and able to activate transcription from GAL4-responsive promoters.
-If the activated promoter controls the transcription of a reporter gene (e.g. Aureobasidin A resistance) you can select for those bait-prey interactions.

Category:

Science & Technology

Tags:

• yeast two-hybrid
• Yeast 2 hybrid
• Matchmaker Gal4
• protein-protein interactions
• Aureobasidin A

[larojiblanca]

learning cell & molecular biology from youtube is exponentially more effective and interesting than reading the textbook

[sblackt]

@larojiblanca Agreed.