How to Stain an SDS-PAGE gel

Your gel just finished running -- what do you do next?

In this video, Dan shows you how to disassemble your SDS-PAGE gel, and how to stain it using either PageBlue, Coomassie, or Silver Staining.

Coomassie is the older method of staining, and gives bright blue bands on the gel. PageBlue is similar, but provides a quicker alternative. If you have faint bands and require a more sensitive method, you can use Silver Staining.

Choose whatever method you need, but beware of the hazardous materials that may be used.

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Category:

Science & Technology

Tags:

• labtricks
• science
• lab
• tips
• tricks
• techniques
• SDS
• PAGE
• sodium
• dodecyl
• polyacrylamide
• gel
• electrophoresis
• staining
• biochemistry
• molecular
• biology
• protein
• video
• tutorials
• pageblue
• coomassie
• brilliant
• blue
• silver
• nitrate

[osmarguz]

where I can buy the pageblue?

[labtricks]

@osmarguz you can buy it directly from the manufacturer, Fermentas, which is now a part of Thermo Fisher. Try looking at their online catalogue for the prices

[DanzQueen]

Oooh yeah, Page Blue is new. Never used it. Must try...

[CherryMoch]

can you make one for western blotting? thanks! :)

[damnation333]

Thanks for sharing this. Never used PageBlue and the KimWipeTrick is great! Thanks ;)