This video was my final project for the Harvard School of Engineering and Applied Sciences REU in 2010.
Research Description: Organization of cells in colony, tumor or tissue development remains an ongoing puzzle in biology. Direct observation of development should offer insight to many ongoing biological questions including embryonic development, cancer and tissue engineering. We developed a battery-powered holographic microscope capable of 3D cellular resolution. It is simple, self-contained, portable, costs less than $1,000.00, and fits with in a one foot cube. We demonstrated its unique capability to observe growth in a standard incubation chamber using yeast as a model system. In the future, we intend to use this microscope to observe and quantify growth rates and cell organization of tumors in three-dimensions.
Special thanks to:
Tom Dimiduk
Vinothan Manoharan
Kathryn Hollar
Brianna Nyberg
Harvard SEAS
NSF funding
"Fluorescence fade the black"...YES
JamesKUtterback 1 year ago