General Biochemistry Review

This course is part of a series taught by Kevin Ahern at Oregon State University on General Biochemistry. For more information about online courses go to http://ecampus.oregonstate.edu/

1. Base excision repair can removed damaged based from DNA. It differs from nucleotide excision repair in removing the damaged base first, followed by removal of a segment where the base was.

2. Disruption of error correction systems can have severe consequences.

3. Error-related systems associated with cancer include HNPCC (colon cancer) and BRC-A (not mentioned in class), which is involved in DNA repair. A critical protein for monitoring DNA for damage prior to division is p53. It can stop the cell cycle if it senses damage and initiate repair. If repair is unable to be performed, p53 can induce cellular suicide - apoptosis.

4. An Ames test uses a selectable marker that can give a readily observable phenotype (such as growth on antibiotic) when mutation happens. By comparing the number of cells with the observable phenotype in a the presence of a test compound to the number of cells in another tube lacking that compound, the mutagenicity of a compound can be determined.

5. Recombination of DNA results in mixing and matching of DNA sequences. The process occurs most often between homologous sequences on different chromosomes. The process can be quite active during meiosis.

6. Recombination proceeds through formation of a Holliday junction. Holliday junctions form as a result of alignment of homologous sequences, followed by cleavage of strands on each chromosome, invasion of the strands into the opposite chromosome, movement of the junction, another cleavage reaction, followed by reformation of phosphodiester bonds.

7. Enzymes involved in recombination are called recombinases and are similar in function to the integrase of HIV.

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[novomlinski]

@DeadStarsShine i'm sure you can find more work with biochemistry...

[prs889]

hmm.. biochemistry is pretty interesting! 

[susannepiige]

'kay?



[Alex110993S]

Why does milk precipitate at pH4 without carbohydrates and does not precipitate at the same pH with carbohydrates?

[DeadStarsShine]

What's better... biochemistry or astrophysics?

[maraqidi]

@Tstorm842

[maraqidi]

@Tstorm842

show respect stupid.

[JohnnyShoulders]

Thank goodness for those Ogazki fragments. I think that is what you call it.

[Tstorm842]

I think this professor's ass is too big for my screen

[MrChem303]

I really love Biochemistry and this video.. but i think that the prof is really scary with his weird hairdo.. really genius... :D