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Gene Expression & Regulation with Tet-On® Advanced Inducible Expression

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Uploaded by on Dec 3, 2009

For more information visit: http://www.clontech.com/US/Products/Inducible_Systems/Tetracycline-Inducible_...

Tet-On Advanced provides on-demand, robust expression of your gene of interest (GOI) in target cells. Target cells that express the Tet-On Advanced transactivator, and that also contain an integrated TRE-based expression vector (e.g., pTRE-Tight) will express high levels of your GOI when cultured in the presence of the system's inducer, doxycycline (Dox). In the presence of Dox, Tet-On Advanced binds tightly and specifically to PTight, and activates high level transcription of the downstream GOI.

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