KESM fluorescence test run

This is a test run of the Knife-Edge Scanning Microscope, going through the tip of the cerebellum (coronal section) of a 2.5-week old GFP mouse brain. The video was taken with Panasonic DMC-TZ3 in movie mode. The green near-vertical line in the middle is the green-filtered light of blue laser hitting near the edge of the diamond knife in the KESM (the laser line is a bit off parallel from the knife tip). As the movie runs, you can see black blobs moving below the knife in the background. This is the brain tissue (it was counter-stained in Nissl, hence the dark color). A slight fluorescence can be observed compared to the backgroud as the knife goes over the tissue area of the specimen block. YC 1/13/2009

Category:

Science & Technology

Tags:

• mouse
• brain
• imaging