Detection of Bacteria and Fungi in Cell Culture

( http://www.abnova.com ) - It' s very important to test cell lines and cell culture media to determine if microbial contamination is present. The culture media used for the tests are tryptic soy broth (TSB) for the detection of aerobic and anaerobic bacteria, and fluid thioglycollate medium (FTM) for the detection of erobes, facultative anaerobes and fungi. More videos at Abnova http://www.abnova.com

Category:

Science & Technology

Tags:

• Detection
• of
• Bacteria
• and
• Fungi
• Cell
• Culture

[GretchunKim]

Bernhardusa: ADD fungicide or bactericide to the cell media (TSB or FTM) and inoculate with the sample in question to identify which one it is. Some bacteria not responsible to broad spectrum bactericide and a lot of times it is not worth the effort to identify the contaminant. What a great question!

[GretchunKim]

When the cell culture get contaminated (depending on the stage [late or early] of the contamination) by fungi, the contaminant look fuzzy with elongated/branched filament or hair-like projections under a light microscope. If it is an early stage bacterial contamination, it is possible to observe black dots--> eventually will outgrow and kill the cells of our interest and make the cell medium cloudy.

[bernhardusa]

Thx for the video. If you have a culture, mixed with bacteria and fungus: How can you determine and extract each group ? I mean, how can you identify and separate the fungus from any other kind of bacteria ?