PCR

PCR

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  Film & Animation

[RareEmerauld86]

It was driving me nuts when he kept saying the term "melting" - You don't melt the DNA dude! DNA is being DENATURED - separation of strands!!!

[caNNoNNboydeNNis]

oh my gosh!! u saved my biology mark :D

[pnocen91]

well, I believe there's one mistake - after 30 cycles, there will be 2^30-2*30=1'073'741'764 target molecules,

We can see at the picture given at time of 4:04 that there is 1'073'741'766, so there are 2 extra molecules given by author.

[knochentrocken96]

It's very nice to see this whole process visualized. Now I REALLY understand it.

[killazerg1]

Melting is the breaking of strands. Think about the phase change from solid to liquid; what chemical changes must take place?

[jrd24545]

ummm you are right...but I understand why he says melting. The temperature required to separate the stands can be called "Melting Temperature" and one of my MCAT books says melted DNA can be a synonymy for denatured DNA.

[88EIIi]

This was very helpful. Thank you!

[HadasLL]

It's true that what's really going on is denaturation, but it seems like 'melting' is the common term for this process during PCR. It's in my course notes as well

[Jen090393]

Love this video, so helpful! Going to be watching this over and over before my exam tomorrow :(

[DhooperTheSuper]

Come down, it's a metaphor used to facilitate learning. Anyways, what is melting? A separation of intermolecular interactions...what's happening here? Stop being so picky, the best scientists draw from multiple sources and aren't so worried using the technically correct terms.

[elhamy abd almonem]

good video for PCR technique

[Steve Dilk]

If only my professor was a supporter of YouTube... And the melting temperature of DNA refers to the point at which the strands dissociate so the primers can anneal to the stands when the temperature is cooled back down. DON'T HATE THE PLAYER HATE THE GAME HOMIE THIS DUDE IS RIGHT!