Supplementary Movie 1

Video of two-channel acquisition during a representative hypoxia
experiment. Left frames are vasculature with dye; right frames are
NAD(P)H fluorescence. Images played at 24x speed, with entire
sequence constituting four minute tracked acquisition. Amount of
motion compensation for this experiment is shown in Figure 5. At t=0,
nitrogen replaces oxygen in the ventilator and the onset of systemic
hypoxia occurs during the first minute. Upper frames show both
channels raw acquisition from tracking microscope, while lower frames
show in-plane motion corrected output from retrospective deformable
image registration algorithm.

Category:

Science & Technology

Tags:

• microscope
• motion
• correction
• multiphoton