Concentrations Part 5 - serial dilution

Fifth video in a series of videos discussing concentration calculations commonly used in a laboratory. More specifically a molecular biology lab. This particular video shows the basics behind setting up and calculating a serial dilution. This procedure can be used to establish a standard curve from a solution of known concentration.

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[Arishtat]

Greate series. I'm a little confused about the total volumes, though. In the end, doesn't well 8 contain a total of 300µl and all other wells 270µl?

[gkpeter]

@Arishtat Yes, so that is why you have to think ahead.  For example if I was using this dilution series for a standard curve and I need triplicates of 100 uL, it wouldn't work.

[summoncaster]

Ty, very helpful. But there is an error in the two last dilution...

[gkpeter]

Your right, thanks. I will annotate that later. Good catch.

[donshan123]

Excuse me Sir...Hey you, Sir!! Umm yeah LOL!!....I just wanted to tell you that you a blessing beyond belief. I am in my micro lab trying to do this for a homework assignment and your video has truly helped me. Thank you so much for posting this.

[piarocio2013]

haha you lost me at final dilution factor... XD

[mypeniscanjetfire]

Thank you very much.

[smartaleck05]

I have a practical test tomorrow, and this really helped! Thanks.

[603fofo]

Thank u so much !!!

[iarue]

Hello nice video. I understand most of it apart from where u talked about the other way of doing it instead of pippeting it out using the serial dilution. Then u mention 1ul of stock/100 L H20. I was wondering if u could explain how u derived those numbers. Thanks

[leaderofpeace]

@Arishtat you are correct to say that the volumes are 300 uL because the dilution is always calculated with the total volume (30 + 270) then that is intern used for the stock

[NappyNaturally]

...thank u

[rocio89]

excellent video!!!! very well explained and very clear!!

I needed to remember how to do this before going to the lab, now I don't have to ask my prof!! Thanks :)

[ashimqc]

thanks

[nareshvasani]

Formula for Dilution factor is Final volume by Aliquot volume.

This is correct or not please let me know.

[e2sont]

Thank you so much now I understand it!