Isolation of peripheral blood mononuclear cells using ficoll-1077.
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not bad but one should NEVER use that kind pipet to recover the cells from the mononuclear layer. I always teach people to use a 1 ml disposable 12" pipet for this! Or, use elongated 10" glass pasteur pipets with a bulb and cotton plug. sterilized of course. NEVER use that P-1000 you used because it is way too short and you are in danger of contaminating the tube, and it is just NOT long enough! :)!
BeaconCampana 1 year ago 2