More informations here (projet BIOSED) : http://bioecologie.over-blog.com/
Continental freshwater sediments act as an accumulation compartment for metallic pollutants as uranium. Our studys main aim is to understand and quantify effects of benthic macroinvertebrates and microorganisms on uranium speciation and fluxes through the water-sediment interface. So, there is an inter-relationship between bioturbation from benthic macroinvertebrates, geochemistry, and micro-organisms communities in the sediment. We will try to answer to this question: which are the different or associated roles of those three parameters on the sediments reactivity and capacity to transfer uranium? Many studies have been realized on bioturbation effects on metal speciation through the interface, or on the micro-organisms communities distribution in the sediment, but not on these two topics both in the same time, and moreover, on natural un-remade sediment. In order to reach our aims, sediment cores were extracted from a uranium contaminated site near abandoned uranium mines (Limousin, France) and installed in laboratory to manipulate two variables: benthic bioturbating macroinvertebrates (Tubifex Tubifex) and microbial communities in the sediment. Sediments geochemistry and uranium bioavailability have been studied by methods such as microelectrodes, DET (diffusive equilibrium thin-films), and DGT (diffusive gradient in thin-films). Concentration profiles were made for iron and manganese in order to compare them to uraniums profiles. Vertical dissolved oxygen distributions were studied thanks to microelectrodes. Fluxes will be calculated through the interface for different experimental conditions (with or without macroinvertebrates or/nor micro-organisms). Bioturbation will be quantified thanks to fluorescent markers. Finally, microbial abundance and diversity will be studied by DGGE method (Denaturing Gradient Gel Electrophoresis).
C'est gentil ! C'est à cause de la fleur en bois derrière que tu dis ça !?!
EchecTheory 1 year ago
ouh là! toutes les filles vont tomber à tes pieds pendant que tu seras occupé à faire tenir les fleurs debout !
mineuf 2 years ago